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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation.
doi: 10.4049/jimmunol.1300989
Figure Lengend Snippet: FIGURE 7. Enhanced in vivo immune responses in DUSP14-deficient (DUSP14-KO) mice. WT and DUSP14-KO mice were immunized with KLH emulsified in alum. Seven days later, enlarged lymph nodes were isolated and restimulated with KLH for 72 h. (A) Cell proliferation was measured using [3H]thymidine incorporation. (B) IL-2, IL-4, and IFN-g levels in supernatants were measured using ELISA. Three mice were an- alyzed for each genotype. Data are mean 6 SEM. (C) WT and DUSP14- KO mice were immunized as before. Sera were collected at 14 d after the primary immunization. NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 Abs were determined by ELISA. Results are presented relative to those of normal serum from a WT mouse. Five mice were analyzed for each ge- notype. Data are mean 6 SEM. *p , 0.05, two-tailed t test.
Article Snippet: The titers of anti–NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 in the sera were measured by ELISAs (eBioscience) using NP-BSA (Biosearch Technologies) as the coating Ag, followed by incubation with anti-IgM, anti-IgG1, anti-IgG2a, anti-IgG2b, and
Techniques: In Vivo, Isolation, Enzyme-linked Immunosorbent Assay, Two Tailed Test